Loading...
Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice.
Bergmann, Silke Beard, Philippa M Pasche, Bastian Lienenklaus, Stefan Gahan, Cormac G M Schughart, Klaus Lengeling, Andreas
Bergmann, Silke
Beard, Philippa M
Pasche, Bastian
Lienenklaus, Stefan
Gahan, Cormac G M
Schughart, Klaus
Lengeling, Andreas
Files
Loading...
Open Access publication
Adobe PDF, 2.85 MB
Other Titles
Abstract
The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of 'murinisation' to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised Listeria strains to examine the spatiotemporal dissemination of Listeria in four diverse mouse genetic backgrounds after oral inoculation.
Citation
Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. 2013, 13:90 BMC Microbiol.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1471-2180
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Archived with thanks to BMC microbiology