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PANCREATIC LIPASE : CRYSTALLIZATION, DOMAIN INVESTIGATION AND CLONING
Chapus, Catherine Abousalham, Karim Kerfelec, Brigitte Moulard, Maxime Foglizzo, Edith Lombardo, Dominique
Chapus, Catherine
Abousalham, Karim
Kerfelec, Brigitte
Moulard, Maxime
Foglizzo, Edith
Lombardo, Dominique
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Issue Date
1991
Submitted date
2024-03-06
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Abstract
Pancreatic lipase is involved in dietary fat digestion. The enzyme, which belongs to a special class of esterases, realizes an heterogeneous catalysis. Although extensively studied for many years, the mechanism of action of lipase on emulsified substrates is still not elucidated. Crystals of man and horse pancreatic lipases suitable for an X-ray investigation have been obtained. The cell parameters of the various crystalline forms have been determined. Heavy atom derivatives are under investigation. The spatial organization of pancreatic lipase in two well defined domains has been studied through limited proteolysis of the enzyme from various species. A horse pancreatic cDNA library has been built and the cDNAs encoding for lipase and colipases isolated.
Citation
Lipases : structure, mechanism and genetic engineering, 35 - 38
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International