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Cell culture-derived HCV cannot infect synovial fibroblasts.
Nadeem, Abd-Elshafy D Thomas, Pietschmann Ulf, Müller-Ladner Elena, Neumann Anggakusuma Mohamed, Bahgat M Frank, Pessler Patrick, Behrendt
Nadeem, Abd-Elshafy D
Thomas, Pietschmann
Ulf, Müller-Ladner
Elena, Neumann
Anggakusuma
Mohamed, Bahgat M
Frank, Pessler
Patrick, Behrendt
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Abstract
Worldwide 170 million individuals are infected with hepatitis C virus (HCV), up to 45 million of whom are affected by arthropathy. It is unclear whether this is due to viral infection of synovial cells or immune-mediated mechanisms. We tested the capacity of primary synovial fibroblasts to support HCV propagation. Out of the four critical HCV receptors, only CD81 was expressed to any significant extent in OASF and RASF. Consistent with this, pseudotyped HCV particles were unable to infect these cells. Permissiveness for HCV replication was investigated by transfecting cells with a subgenomic replicon of HCV encoding a luciferase reporter. OASF and RASF did not support replication of HCV, possibly due to low expression levels of miR-122. In conclusion, primary human synovial fibroblasts are unable to support propagation of HCV in vitro. HCV-related arthropathy is unlikely due to direct infection of these cells.
Citation
Cell culture-derived HCV cannot infect synovial fibroblasts. 2015, 5:18043 Sci Rep
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Journal
PubMed ID
PubMed Central ID
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Article
Language
en
Description
Series/Report no.
ISSN
2045-2322