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Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity.
Becker, Matthias Strengert, Monika Junker, Daniel Kaiser, Philipp D Kerrinnes, Tobias Traenkle, Bjoern Dinter, Heiko Häring, Julia Ghozzi, Stéphane Zeck, Anne
Becker, Matthias
Strengert, Monika
Junker, Daniel
Kaiser, Philipp D
Kerrinnes, Tobias
Traenkle, Bjoern
Dinter, Heiko
Häring, Julia
Ghozzi, Stéphane
Zeck, Anne
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2021-02-19
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Abstract
The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis is required to understand the manifestation and progression of COVID-19, monitor seroconversion within the general population, and support vaccine development. The majority of currently available commercial serological assays only quantify the SARS-CoV-2 antibody response against individual antigens, limiting our understanding of the immune response. To overcome this, we have developed a multiplex immunoassay (MultiCoV-Ab) including spike and nucleocapsid proteins of SARS-CoV-2 and the endemic human coronaviruses. Compared to three broadly used commercial in vitro diagnostic tests, our MultiCoV-Ab achieves a higher sensitivity and specificity when analyzing a well-characterized sample set of SARS-CoV-2 infected and uninfected individuals. We find a high response against endemic coronaviruses in our sample set, but no consistent cross-reactive IgG response patterns against SARS-CoV-2. Here we show a robust, high-content-enabled, antigen-saving multiplex assay suited to both monitoring vaccination studies and facilitating epidemiologic screenings for humoral immunity towards pandemic and endemic coronaviruses.
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Nat Commun. 2021 Mar 11;12(1):1577. doi: 10.1038/s41467-021-21609-2.
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en
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2041-1723