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Cystobactamid 507: Concise Synthesis, Mode of Action and Optimization toward More Potent Antibiotics.
Elgaher, Walid A M Hamed, Mostafa M Baumann, Sascha Herrmann, Jennifer Siebenbürger, Lorenz Krull, Jana Cirnski, Katarina Kirschning, Andreas Müller, Rolf
Elgaher, Walid A M
Hamed, Mostafa M
Baumann, Sascha
Herrmann, Jennifer
Siebenbürger, Lorenz
Krull, Jana
Cirnski, Katarina
Kirschning, Andreas
Müller, Rolf
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2020-01-26
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Lack of new antibiotics and increasing antimicrobial resistance are the main concerns of healthcare community nowadays, which necessitate the search for novel antibacterial agents. Recently, we discovered the cystobactamids - a novel natural class of antibiotics with broad-spectrum antibacterial activity. In this work, we describe a concise total synthesis of cystobactamid 507, the identification of the bioactive conformation using non-covalently bonded rigid analogs, the first structure–activity relationship (SAR) study for cystobactamid 507 leading to new analogs with high metabolic stability, superior topoisomerase IIA inhibition, antibacterial activity and, importantly, stability toward the resistant factor AlbD. Deeper insight into the mode of action revealed that the cystobactamids employ DNA minor groove binding as part of the drug–target interaction without showing significant intercalation. By designing a new analog of cystobactamid 919-2 we finally demonstrated that these findings could be further exploited to obtain more potent hexapeptides against Gram-negative bacteria.
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Chemistry. 2020 Jan 26. doi: 10.1002/chem.202000117.
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en
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1521-3765
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Attribution-NonCommercial-ShareAlike 4.0 International