Loading...
Salmonella Typhimurium discreet-invasion of the murine gut absorptive epithelium.
Fattinger, Stefan A Böck, Desirée Di Martino, Maria Letizia Deuring, Sabrina Samperio Ventayol, Pilar Ek, Viktor Furter, Markus Kreibich, Saskia Bosia, Francesco Müller-Hauser, Anna A
Fattinger, Stefan A
Böck, Desirée
Di Martino, Maria Letizia
Deuring, Sabrina
Samperio Ventayol, Pilar
Ek, Viktor
Furter, Markus
Kreibich, Saskia
Bosia, Francesco
Müller-Hauser, Anna A
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2020-05-04
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 5.54 MB
Other Titles
Abstract
Salmonella enterica serovar Typhimurium (S.Tm) infections of cultured cell lines have given rise to the ruffle model for epithelial cell invasion. According to this model, the Type-Three-Secretion-System-1 (TTSS-1) effectors SopB, SopE and SopE2 drive an explosive actin nucleation cascade, resulting in large lamellipodia- and filopodia-containing ruffles and cooperative S.Tm uptake. However, cell line experiments poorly recapitulate many of the cell and tissue features encountered in the host's gut mucosa. Here, we employed bacterial genetics and multiple imaging modalities to compare S.Tm invasion of cultured epithelial cell lines and the gut absorptive epithelium in vivo in mice. In contrast to the prevailing ruffle-model, we find that absorptive epithelial cell entry in the mouse gut occurs through "discreet-invasion". This distinct entry mode requires the conserved TTSS-1 effector SipA, involves modest elongation of local microvilli in the absence of expansive ruffles, and does not favor cooperative invasion. Discreet-invasion preferentially targets apicolateral hot spots at cell-cell junctions and shows strong dependence on local cell neighborhood. This proof-of-principle evidence challenges the current model for how S.Tm can enter gut absorptive epithelial cells in their intact in vivo context.
Citation
PLoS Pathog. 2020 May 4;16(5):e1008503. doi: 10.1371/journal.ppat.1008503.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
EISSN
1553-7374
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution 4.0 International