Loading...
Murine solid tumours as a novel model to study bacterial biofilm formation in vivo.
Pawar, V Crull, K Komor, U Kasnitz, N Frahm, M Kocijancic, D Westphal, K Leschner, S Wolf, K Loessner, H
Pawar, V
Crull, K
Komor, U
Kasnitz, N
Frahm, M
Kocijancic, D
Westphal, K
Leschner, S
Wolf, K
Loessner, H
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2014-08
Submitted date
Files
Loading...
original manuscript
Adobe PDF, 212.68 KB
Loading...
figures to manuscript
Adobe PDF, 4.61 MB
Other Titles
Abstract
Bacteria of many species are able to invade and colonize solid tumours in mice. We have focused on Salmonella enterica serovar Typhimurium. Detailed analysis revealed that such tumour-invading Salmonella form biofilms, thus providing a versatile in vivo test system for studying bacterial phenotypes and host-pathogen interactions. It appears that biofilm formation by S. typhimurium is induced as a defence against the immune system of the host, and in particular against neutrophils. Further, we extended our work to the clinically more relevant biofilm infection by Pseudomonas aeruginosa. The induction of P. aeruginosa biofilms in neoplastic tissue appears to be elicited as a reaction against the immune system. Reconstitution experiments reveal that T cells are responsible for biofilm induction. Isogenic mutants that are no longer able to form biofilms can be used for comparison studies to determine antimicrobial resistance, especially therapeutic efficacy against P. aeruginosa located in biofilms.
Citation
Murine solid tumours as a novel model to study bacterial biofilm formation in vivo. 2014, 276 (2):130-9 J. Intern. Med.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1365-2796