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Importance of flagella in acute and chronic Pseudomonas aeruginosa infections.

Lorenz, Anne
; 
Preuße, Matthias
; 
Bruchmann, Sebastian
; 
Pawar, Vinay
; 
Grahl, Nora
; 
Pils, Marina C
; 
Nolan, Laura M
; 
Filloux, Alain
; ; 
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Issue Date
2018-11-08
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original manuscript
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supplementary figures
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supplementary table S1
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supplementary table S2
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supplementary table S3
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Abstract
Pseudomonas aeruginosa is an environmental microorganism and a causative agent of diverse acute and chronic, biofilm-associated infections. Advancing research-based knowledge on its adaptation to conditions within the human host is bound to reveal novel strategies and targets for therapeutic intervention. Here, we investigated the traits that P. aeruginosa PA14 as well as a virulence attenuated ΔlasR mutant need to survive in selected murine infection models. Experimentally, the genetic programs that the bacteria use to adapt to biofilm-associated versus acute infections were dissected by passaging transposon mutant libraries through mouse lungs (acute) or mouse tumours (biofilm-infection). Adaptive metabolic changes of P. aeruginosa were generally required during both infection processes. Counter-selection against flagella expression was observed during acute lung infections. Obviously, avoidance of flagella-mediated activation of host immunity is advantageous for the wildtype bacteria. For the ΔlasR mutant, loss of flagella did not confer a selective advantage. Apparently, other pathogenesis mechanisms are active in this virulence attenuated strain. In contrast, the infective process of P. aeruginosa in the chronic biofilm model apparently required expression of flagellin. Together, our findings imply that the host immune reactions against the infectious agent are very decisive for acuteness and duration of the infectious disease. They direct disease outcome.
Citation
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Wiley-Blackwell
Journal
URI
http://hdl.handle.net/10033/621615
DOI
10.1111/1462-2920.14468
PubMed ID
30411474
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1462-2920
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Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the research group of molecular bacteriology(MOBA)
publications of the scientific administration (GFW)
publications of the service unit animal experimentation: (TEE)