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RNA polymerase motions during promoter melting.
Feklistov, Andrey Bae, Brian Hauver, Jesse Lass-Napiorkowska, Agnieszka Kalesse, Markus Glaus, Florian Altmann, Karl-Heinz Heyduk, Tomasz Landick, Robert Darst, Seth A
Feklistov, Andrey
Bae, Brian
Hauver, Jesse
Lass-Napiorkowska, Agnieszka
Kalesse, Markus
Glaus, Florian
Altmann, Karl-Heinz
Heyduk, Tomasz
Landick, Robert
Darst, Seth A
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2017-05-26
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All cellular RNA polymerases (RNAPs), from those of bacteria to those of man, possess a clamp that can open and close, and it has been assumed that the open RNAP separates promoter DNA strands and then closes to establish a tight grip on the DNA template. Here, we resolve successive motions of the initiating bacterial RNAP by studying real-time signatures of fluorescent reporters placed on RNAP and DNA in the presence of ligands locking the clamp in distinct conformations. We report evidence for an unexpected and obligatory step early in the initiation involving a transient clamp closure as a prerequisite for DNA melting. We also present a 2.6-angstrom crystal structure of a late-initiation intermediate harboring a rotationally unconstrained downstream DNA duplex within the open RNAP active site cleft. Our findings explain how RNAP thermal motions control the promoter search and drive DNA melting in the absence of external energy sources.
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RNA polymerase motions during promoter melting. 2017, 356 (6340):863-866 Science
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en
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1095-9203
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openAccess