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THE USE OF FLUORESCENCE SENSORSAS OPTICAL BIOSENSORS
Schelp, C. Scheper, T. Bückmann, A. F.
Schelp, C.
Scheper, T.
Bückmann, A. F.
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
The use of miniaturized fluorescence probes for on-line monitoring analytes in continuous- flow systems (automated clinical analysis, process control, e.g. in biotechnological processes, environmental control, etc.) has becomeincreasingly important. Wereport the application of a fiber-optic based fluorescence probe used as optrode for a novel optical biosensor system to monitor NAD(H) dependent enzymatic reactions based on the flow-injection analysis (FIA) principle. A miniaturized fiber-optic fluorimeter is combined with a small size membrane reactor (1 ml) (Fig. 1). In here, enzymes (dehydrogenases) for analyte conversion and coenzymeregeneration as well as the macromolecular coenzyme derivative polyethyleneglycol (PEG, M, = 20,000)-N°-(2-aminoethyl)-NAD(H) [1] are retained in the direct vicinity of the sensor tip of a concentric fiber-optic probe (fluorescence excitation 360 nm / emission 460 nm) by an ultrafiltration membrane (cutoff: 5000 Dalton) (Fig. 2). Only low molecular-weight compounds (analytes, products, etc.) can freely pass through thisultrafiltration membrane.
Citation
Biosensors : applications in medicine, environmental protection and process control, 263 - 266
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Type
Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International