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Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells.

Somplatzki, Stefan
; 
Mühlenhoff, Martina
; ; 
Gerardy-Schahn, Rita
; 
Böldicke, Thomas
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2017-05-12
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Abstract
Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown. To inhibit the function of ST8SiaII and ST8SiaIV specific ER IBs were generated starting from two corresponding hybridoma clones. Both IBs αST8SiaII-IB and αST8SiaIV-IB were constructed in the scFv format and their functions characterized in vitro and in vivo.
Citation
Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells. 2017, 17 (1):42 BMC Biotechnol.
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Helmholtz Centr for infection research
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BMC biotechnology
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http://hdl.handle.net/10033/620928
DOI
10.1186/s12896-017-0360-7
PubMed ID
28499450
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Language
en
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1472-6750
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publications of the research group immunity and infection (IMMI)
Publications of the research group structure and functions of Proteins(SFPR)