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FLOW INJECTION MONITORING OF ENZYME REACTIONS ON SOLID SURFACES

Christian, Gary D.
; 
Ruzicka, Jaromir
; 
Chow, Kingsum
; 
Chung, Suzanne
; 
Yerian, Tracy D.
; 
Scampavia, Louis D.
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
Optosensing flow injection analysis may be used to monitor enzyme reactionson various types of solid surfaces, e.g., cellulose, Teflon or polypropylene. The surfaces may bein the form ofa pad, a membraneorparticles (in a column). While the enzyme maybe in solution, advantages accrueif it is attached to the solid surface. Traditional enzyme immobilization on surfaces involves chemical bonding reactions, but enzymes may be directly adsorbed onto certain hydrophobic surfaces. The principles of optosensing measurements are presented andapplicationsto cellulose pads and gas barrier membranes for enzyme measurements are reviewed. Recent novel methodsfor derivatizing enzymes for direct adsorption onto fluorocarbon membranesare presented. A new gas sensing (gas gap) membrane is described in which the membraneacts as the actual sensor and notjust a barrier, suitable for optosensing of monitoring enzyme reactions. Preliminary studies of the direct adsorption of urease enzyme onto the membrane are presented. Silica based C-18 particles in a microcolumn are used to adsorb an indicator and native urease enzymé. Optosensing measurementsof urea by measuring transmittance through the particles are presented.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 267 - 276
Affiliation
Department of Chemistry, BG-10, University of Washington, Seattle, WA 98195 USA
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991
URI
http://hdl.handle.net/10033/623640
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Type
Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
1560811218
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International
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