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Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion.

De Vrieze, Jo
; 
Regueiro, Leticia
; 
Props, Ruben
; ; 
Jáuregui, Ruy
; 
Pieper, Dietmar H
; 
Lema, Juan M
; 
Carballa, Marta
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2016
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Abstract
The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on DNA (total community) and RNA (active community) based on the 16S rRNA (gene) with respect to salt concentration and response time was evaluated.
Citation
Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion. 2016, 9:244 Biotechnol Biofuels
Affiliation
HelmholtzCentre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
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Biotechnology for biofuels
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http://hdl.handle.net/10033/620642
DOI
10.1186/s13068-016-0652-5
PubMed ID
27843490
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en
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publications of the work group microbial interactions and processes (MINP)