Loading...
RNA structure determination by solid-state NMR spectroscopy.
Marchanka, Alexander Simon, Bernd Althoff-Ospelt, Gerhard Carlomagno, Teresa
Marchanka, Alexander
Simon, Bernd
Althoff-Ospelt, Gerhard
Carlomagno, Teresa
Files
Loading...
Open Access Publication
Adobe PDF, 1.24 MB
Other Titles
Abstract
Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize—and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.
Citation
RNA structure determination by solid-state NMR spectroscopy. 2015, 6:7024 Nat Commun
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
2041-1723