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A STRATEGY FOR ISOLATION AND STRUCTURAL ANALYSIS OF GLYCOPROTEIN OLIGOSACCHARIDES
Angel, Anne-Sophie Grönberg, Gunnar Nilsson, Göran Strömberg, Stefan Nilsson, Bo
Angel, Anne-Sophie
Grönberg, Gunnar
Nilsson, Göran
Strömberg, Stefan
Nilsson, Bo
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Issue Date
1991
Submitted date
2024-02-20
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Adobe PDF, 4.82 MB
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Abstract
A general strategy is described for release, separation and structural analysis of Nand O-linked glycans from glycoproteins. Following sugar analysis, to determine the monosaccharide composition and the total carbohydrate content, the glycoprotein is degraded by chemical and/or enzymatic methods. The N- and O-linked glycans are separated and further fractionated into pure compoundsby gelfiltration, affinity chromatography and high performance ion exchange chromatography. Structural analysis is carried out by chemical analyses, fast atom bombardment mass spectrometry and 500 MHz 1H-NMR spectroscopy. Complete structural analysis is obtained of each glycan as well as determination of the glycosylationsites.
Citation
Protein glycosylation, 125 - 135
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 15
ISSN
0930-4320
EISSN
ISBN
3527283676
1560811846
1560811846
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Attribution-NonCommercial-ShareAlike 4.0 International