Loading...
Molecular dissection of Salmonella-induced membrane ruffling versus invasion.
Hänisch, Jan Ehinger, Julia Ladwein, Markus Rohde, Manfred Derivery, Emmanuel Bosse, Tanja Steffen, Anika Bumann, Dirk Misselwitz, Benjamin Hardt, Wolf-Dietrich
Hänisch, Jan
Ehinger, Julia
Ladwein, Markus
Rohde, Manfred
Derivery, Emmanuel
Bosse, Tanja
Steffen, Anika
Bumann, Dirk
Misselwitz, Benjamin
Hardt, Wolf-Dietrich
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2010-01
Submitted date
Files
Loading...
original manuscript
Adobe PDF, 1.27 MB
Other Titles
Abstract
Type III secretion system-mediated injection of a cocktail of bacterial proteins drives actin rearrangements, frequently adopting the shape of prominent protuberances of ruffling membrane, and culminating in host cell invasion of Gram-negative pathogens like Salmonella typhimurium. Different Salmonella effectors are able to bind actin and activate Rho-family GTPases, which have previously been implicated in mediating actin-dependent Salmonella entry by interacting with N-WASP or WAVE-complex, well-established activators of the actin nucleation machine Arp2/3-complex. Using genetic deletion and RNA interference studies, we show here that neither individual nor collective removal of these Arp2/3- complex activators affected host cell invasion as efficiently as Arp2/3-complex knock-down, although the latter was also not essential. However, interference with WAVE-complex function abrogated Salmonella-induced membrane ruffling without significantly affecting entry efficiency, actin or Arp2/3-complex accumulation. In addition, scanning electron microscopy images captured entry events in the absence of prominent membrane ruffles. Finally, localization and RNA interference studies indicated a relevant function in Salmonella entry for the novel Arp2/3-complex regulator WASH. These data establish for the first time that Salmonella invasion is separable from bacteria-induced membrane ruffling, and uncover an additional Arp2/3-complex activator as well as an Arp2/3-complex-independent actin assembly activity that contribute to Salmonella invasion.
Citation
Molecular dissection of Salmonella-induced membrane ruffling versus invasion. 2010, 12 (1):84-98 Cell. Microbiol.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1462-5822