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Characterization and role of a metalloprotease induced by chitin in Serratia sp. KCK.

Kim, Hyun-Soo
; 
Golyshin, Peter N
; 
Timmis, Kenneth N
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2007-11
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Abstract
A metalloprotease induced by chitin in a new chitinolytic bacterium Serratia sp. Strain KCK was purified and characterized. Compared with other Serratia enzymes, it exhibited a rather broad pH activity range (pH 5.0-8.0), and thermostability. The cognate ORF, mpr, was cloned and expressed. Its deduced amino acid sequence showed high similarity to those of bacterial zinc-binding metalloproteases and a well-conserved serralysin family motif. Pretreatment of chitin with the Mpr protein promoted chitin degradation by chitinase A, which suggests that Mpr participates in, and facilitates, chitin degradation by this microorganism.
Citation
Characterization and role of a metalloprotease induced by chitin in Serratia sp. KCK. 2007, 34 (11):715-21 J. Ind. Microbiol. Biotechnol.
Affiliation
Department of Environmental Microbiology, The Helmholtz Center for Infection Research, Braunschweig, Germany. hyun1006@korea.ac.kr
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Journal
Journal of industrial microbiology & biotechnology
URI
http://hdl.handle.net/10033/19693
DOI
10.1007/s10295-007-0245-1
PubMed ID
17668255
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Article
Language
en
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1367-5435
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Publications of RG Environmental Microbiology (UMW)