Loading...
DEVELOPMENT AND EVALUATION OF BIOSENSORS FOR HIV-SEROLOGY
Aberl, Franz Wolf, Hans Woias, Peter Koch, Sabine Kößlinger, Conrad Drost, Stephan
Aberl, Franz
Wolf, Hans
Woias, Peter
Koch, Sabine
Kößlinger, Conrad
Drost, Stephan
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1992
Submitted date
2024-04-17
Files
Loading...
PDF
Adobe PDF, 2.56 MB
Other Titles
Abstract
During the last decade a new generation of sensors, the biosensors, has been developed for the quantitative measurement of biological substances. Application and specifity of a biosensor are determined by the recognizing biological/biochemical layer and the type of transducer. The specific interactions between enzymes and their substrates, antibodies and antigens or between receptors and hormones are responsible for the selective binding of biological components in solution. In serological diagnosis the rapid and sensitive detection of a viral antigen or the induced immunological response is ofvital interest. Other fields, such as the utilization in production and control processes, extend the spectrum of possible applications for such sensor systems. The HIV-system has been chosen as a model system. A peptide antigen from the p24 core protein and mu- rine monoclonalantibodies were used forinitial experiments. Experiments under morerealistic conditions were performed with a consensus peptide from the hypervariable V3-loop of the HIV-virus and the reactive rabbit immunosera. Ionsensitive fieldeffect transistors and piezoelectric erystals were tested with respect to their suitability for the detection of antibodies. These immunosensors have to be evaluated and optimized with regard to their turn-over periods, sensitivity and cross-selectivity underexactly defined conditions, Theuse of a sensor system based on a biosensor generally possesses - in comparison to conventional analytical systems - advantageslike reduced influence of environmentalinterferences, short response times, and the possibility to develop “intelligent sensors" with the help of integrated signal processing.
Citation
Biosensors : fundamentals, technologies and applications, 123 - 127
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
3527284370
1560812206
1560812206
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International