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The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.
Poblete-Castro, Ignacio Escapa, Isabel F Jäger, Christian Puchalka, Jacek Lam, Carolyn Ming Chi Schomburg, Dietmar Prieto, María Auxiliadora Martins dos Santos, Vítor A P
Poblete-Castro, Ignacio
Escapa, Isabel F
Jäger, Christian
Puchalka, Jacek
Lam, Carolyn Ming Chi
Schomburg, Dietmar
Prieto, María Auxiliadora
Martins dos Santos, Vítor A P
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Abstract
Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.
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The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. 2012, 11:34 Microb. Cell Fact.
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1475-2859
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