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Single-cell transcriptional profiling of splenic fibroblasts reveals subset-specific innate immune signatures in homeostasis and during viral infection.
Pezoldt, Joern Wiechers, Carolin Erhard, Florian Rand, Ulfert Bulat, Tanja Beckstette, Michael Brendolan, Andrea Huehn, Jochen Mueller, Mathias
Pezoldt, Joern
Wiechers, Carolin
Erhard, Florian
Rand, Ulfert
Bulat, Tanja
Beckstette, Michael
Brendolan, Andrea
Huehn, Jochen
Mueller, Mathias
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2021-12-02
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Abstract
Our understanding of the composition and functions of splenic stromal cells remains incomplete. Here, based on analysis of over 20,000 single cell transcriptomes of splenic fibroblasts, we characterized the phenotypic and functional heterogeneity of these cells in healthy state and during virus infection. We describe eleven transcriptionally distinct fibroblastic cell clusters, reassuring known subsets and revealing yet unascertained heterogeneity amongst fibroblasts occupying diverse splenic niches. We further identify striking differences in innate immune signatures of distinct stromal compartments in vivo. Compared to other fibroblasts and to endothelial cells, Ly6C+ fibroblasts of the red pulp were selectively endowed with enhanced interferon-stimulated gene expression in homeostasis, upon systemic interferon stimulation and during virus infection in vivo. Collectively, we provide an updated map of fibroblastic cell diversity in the spleen that suggests a specialized innate immune function for splenic red pulp fibroblasts.
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Commun Biol. 2021 Dec 2;4(1):1355. doi: 10.1038/s42003-021-02882-9.
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en
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2399-3642
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Attribution 4.0 International