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Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility.

Bense, Sarina
; 
Bruchmann, Sebastian
; 
Steffen, Anika
; ; ; 
Düvel, Juliane
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Issue Date
2019-03-12
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Abstract
The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to elucidate the general control mechanisms operating during bacterial growth, we examined the spatiotemporal activity of FlgZ. We re-annotated the P. aeruginosaflgZ open reading frame and demonstrated that FlgZ-mediated downregulation of motility is fine-tuned via three independent mechanisms. First, we found that flgZ gene is transcribed independently from flgMN in stationary growth phase to increase FlgZ protein levels in the cell. Second, FlgZ localizes to the cell pole upon c-di-GMP binding and third, we describe that FimV, a cell pole anchor protein, is involved in increasing the polar localized c-di-GMP bound FlgZ to inhibit both, swimming and swarming motility. Our results shed light on the complex dynamics and spatiotemporal control of c-di-GMP-dependent bacterial motility phenotypes and on how the polar anchor protein FimV, the motor brake FlgZ and the stator proteins function to repress flagella-driven swimming and swarming motility.
Citation
Mol Microbiol. 2019 Mar 12. doi: 10.1111/mmi.14236.
Affiliation
HZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig Germany.
Publisher
Wiley-Blackwell
Journal
Molecular microbiology
URI
http://hdl.handle.net/10033/621763
DOI
10.1111/mmi.14236
PubMed ID
30864240
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Article
Language
en
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ISSN
1365-2958
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Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the research group cell biology (ZBIO)
publications of the research group of molecular bacteriology(MOBA)