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Biosensors for Choline, Choline Esters and Inhibitors of Choline Esterase
Wollenberger, Ulla Löffler, U. Gruß, R. Göpel, W. Scheller, Frieder W.
Wollenberger, Ulla
Löffler, U.
Gruß, R.
Göpel, W.
Scheller, Frieder W.
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Issue Date
1992
Submitted date
2024-04-10
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Abstract
A choline electrode has been developped by coupling immobilized choline oxidase to a hydrogen peroxide electrode. The coimmobilization of cholinesterase permits the measurementof acetylcholine. The excess of both enzymes results in diffusion limited electrode response for both substrates, with relative sensitivities 1: 0.6 for choline if compared with acetylcholine. When kinetically controlled bienzyme sensor with a low activity of cholinesteraseis used, a dimished sensitivity is obtained for acetylcholine with an increased sensitivity for inhibitors, such as NaF, butoxycarboxime,trichlorfon, or dimethoate. Potentially disposable sensors for those inhibitors have been.constructed with cholinesterase coimmobilized with choline oxidase in a gelatin membraneon a platinum electrode and with cholinesterase immobilized in polyurethane on a thick-film metallized platinum electrode. The decreased formation rate of thiocompoundsfrom thiocholinesters serves as measurefor the inhibited enzymein the latter setup.
Citation
Biosensors : fundamentals, technologies and applications, 35 - 41
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International