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PRODUCTION OF LIPASE BY SPOROTRICHUM (CHRYSOSPORIUM) THERMOPHILE APINIS IMMOBILIZED IN ALGINATE AND POROUS GLASS BEADS
Johri, B. N. Alurralde, J. L. Kressdorf, B. Klein, J.
Johri, B. N.
Alurralde, J. L.
Kressdorf, B.
Klein, J.
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Issue Date
1991
Submitted date
2024-03-27
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Abstract
Spores and mycelia of Sporotrichum (Chrysosporium) thermophile Apinis were immobilized in alginate and porous glass beads for lipase production in batch replacement culture and a column reactor. Young mycelia immobilized in alginate produced higher levels of lipase than free cells or spores immobilized in alginate or glass beads. Alginate beads were successfully used in batch culture for more than 300 h without any signs of breakage and little cell washout. Porous glass beads proved a better carrier for spore derived inocula but activity levels were low. Crude lipase of S. thermophile was stable upto 90°C both in free and immobilized state and thus requires further characterization.
Citation
Lipases : structure, mechanism and genetic engineering, 353 - 359
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International