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NEW DEVELOPMENTS IN THE FIELD OF BIOSENSORS- APPLIED TO THE DETERMINATION OF PESTICIDES IN WATER

Bilitewski, Ursula
; 
Beyerdorf-Radeck, B.
; 
Bier, F. F.
; 
Kindervater, Ralf
; 
Krämer, P.
; 
Rüger, P.
; 
Schmid, Rolf D.
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Issue Date
1992
Submitted date
2024-03-17
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Abstract
The application of new methods and biological components to the determination of pesticides in water is described. Triazines were monitored by competitive immunochemical assays applied to flow-through systems. With a FILA, which was based onthe principle of an ELISA, determination of pesticides within the limits of the European drinking water act was possible without preconcentration of the sample. Alternatively, a fluorescence-labelled antibody was used andthe level of binding measured using the method of the evanescent wave technique. With this system a regeneration was possible up to 300 times without anyloss ofactvity. The determination of particular pesticides was supplemented by the development of biosensors for a class of pesticides. This was achieved by microbial sensors for chlorinated compounds and by a test based on the inhibition of cholinesterase by carbamates and organophosphates. The microbial cells of Alcaligenes eutrophus JMP 134 were immobilized on an oxygen electrode, an increase of oxygen uptake being observed in the presence of 2, 4-dichlorophenoxyacetic acid and its derivatives. The inhibition of cholinesterase was monitored either automatically by a flow injection system or by a disposable sensor madebythick film technology.
Citation
Biosensors : fundamentals, technologies and applications, 189 - 197
Affiliation
GBF, Project Group Biosensors, Mascheroder Weg 1, 3300 Braunschweig, FRG; present address: Inst. f. Physikal. und Theoret. Chemie, Univ. Tübingen, Auf der Morgenstelle 8, 7400 Tübingen, FRG; present address: Dep. of Entomology, University of California, Davis, California 95616, USA
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623788
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Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
3527284370
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International
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