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A MICROBIAL SENSOR FOR BOD

Riedel, K.
Alexiev, U.
Neumann, B.
Kuehn, M.
Renneberg, Reinhard
Scheller, Frieder
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Issue Date
1989
Submitted date
2023-12-06
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Abstract
Biochemical oxygen demand (BOD) is a widely used parameter for the determination of biodegradable organic compounds in waste water. The conventional BOD test takes 5 days (BODs) and is thus unsuitable for process control. A more rapid estimation of BOD may be possible by using a microbial sensor containing whole celis immobilized on an oxygen electrode. The first report of such a microbial BOD sensor was published in 1977 by Karube et al. £27. In contrast to microbial sensors for substrate measurements, which should be highly specific, the microorganisms for BOD sensors must have a broad substrate spectrum. Activated sludges obtained from waste water treatment Plants, which contain a variety of species of microorganisms have been used in BOD sensors [1,2]. However, it was difficult to prepare reproducibly working sensors with activated sludge containing mixed Populations [3]. Sensors using pure cultures of microorganisms seem to be more suitable, as has been demonstrated with Trichosporon cutaneum (3-51, Hansenula anomala I[61, Clostridium butyricum [il], Escherichia coli [71 and Bacillus subtilis [5]. Most BOD sensors employing an stationary state measurement exhibit response times of 15 — 20 min.
Citation
Biosensors : applications in medicine, environmental protection and process control, 71 - 74
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Type
Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International