Loading...
USTILAGO MAYDIS LIPOLYTIC ENZYMES: CHARACTERIZATION AND PARTIAL PURIFICATION
Lang, S. Katsiwela, E. Kleppe, F. Wagner, Fritz
Lang, S.
Katsiwela, E.
Kleppe, F.
Wagner, Fritz
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1991
Submitted date
2024-03-27
Files
Loading...
PDF
Adobe PDF, 1.74 MB
Other Titles
Abstract
The crude lipase preparation of Ustilago maydis ATCC 14826 (after growth on coconut oil) was studied with respect to hydrolysis and esterification potential, resp., as well as to purification of lipolytic enzymes. Concerning substrate specifity (hydrolysis) among triglycerides short chain substances were cleaved to an higher extent than long chain or unsaturated compounds. Obvious inhibition of lipase activity was observed when additional amounts of linoleic or linolenic acid were used during hydrolysis of coconut oil. After Amberlite XAD-2 immobilization and transfer into n-hexane the wax ester synthesis potential was confirmed. The purification of crude lipase preparation by chromatographic methods led to two lipolytic enzymes.
Citation
Lipases : structure, mechanism and genetic engineering, 361 - 364
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
EISSN
ISBN
156081165X
3527283323
3527283323
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International