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NOVEL COVALENT AVIDIN IMMOBILIZATION ON GLASSY CARBON ELECTRODES USING A BIFUNCTIONAL REAGENT
Achtnich, U. Tiefenauer, L.
Achtnich, U.
Tiefenauer, L.
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Issue Date
1992
Submitted date
2024-05-08
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Abstract
It is generally accepted that the stability and performance of a biosensor is determined by the way biocomponents are immobilized on the inorganic support. We present a covalent coupling method of the anchor protein avidin to a glassy carbon electrode surface using the heterobifunctional reagent m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS). Thus, a nearly complete, biologically active monolayer of avidin was obtained which is suitable for the immobilization of any biotinylated compound. These modified electrodes are potentially useful as amperometric biosensors.
Citation
Biosensors : fundamentals, technologies and applications, 527 - 530
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International