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BIOREACTOR SYSTEM FOR CLINICAL ANALYSIS
Murachi, Takashi Tabata, Masayoshi Totani, Masayuki Endo, Jiro
Murachi, Takashi
Tabata, Masayoshi
Totani, Masayuki
Endo, Jiro
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Issue Date
1987
Submitted date
2023-06-19
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Abstract
The usefulness of enzymes as the reagents for clinical analysis has been well documented. The enzymes can be used not only in the form of solution but also in the form of immobilized enzyme reactor. A columntype reactor can be readily applied to any continuous flow analytic system. Combined use of flow injection and chemiluminometric detection makes it possible to minimize the sample volume and the analysis time. Two or three different kinds of immobilized enzymes can be aligned in a minicolumn reactor so that the sequential enzymatic reactions be carried out from upstream to down stream without noticeable backward flow of the solutes. Typical examples of the applicability of such bioreactors to clinical analysis are presented. The determination of serum creatinine by sequentially aligned immobilized glutamate dehydrogenase and creatinine deiminase is illustrated. A novel chemiluminometric determination of ammonia by successive passage of the analyte through immobilized urease, glutamate dehydrogenase, and glutamate oxidase is also described.
Citation
Biosensors International Workshop 1987, 119 - 124
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
EISSN
ISBN
0-89573-683-7
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License
Attribution-NonCommercial-ShareAlike 4.0 International