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In Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa.
Pawar, Vinay Komor, Uliana Kasnitz, Nadine Bielecki, Piotr Pils, Marina C Gocht, Benjamin Moter, Annette Rohde, Manfred Weiss, Siegfried Häussler, Susanne
Pawar, Vinay
Komor, Uliana
Kasnitz, Nadine
Bielecki, Piotr
Pils, Marina C
Gocht, Benjamin
Moter, Annette
Rohde, Manfred
Weiss, Siegfried
Häussler, Susanne
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2015-08
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Abstract
Patients suffering from cystic fibrosis (CF) are commonly affected by chronic Pseudomonas aeruginosa biofilm infections. This is the main cause for the high disease severity. In this study, we demonstrate that P. aeruginosa is able to efficiently colonize murine solid tumors after intravenous injection and to form biofilms in this tissue. Biofilm formation was evident by electron microscopy. Such structures could not be observed with transposon mutants, which were defective in biofilm formation. Comparative transcriptional profiling of P. aeruginosa indicated physiological similarity of the bacteria in the murine tumor model and the CF lung. The efficacy of currently available antibiotics for treatment of P. aeruginosa-infected CF lungs, such as ciprofloxacin, colistin, and tobramycin, could be tested in the tumor model. We found that clinically recommended doses of these antibiotics were unable to eliminate wild-type P. aeruginosa PA14 while being effective against biofilm-defective mutants. However, colistin-tobramycin combination therapy significantly reduced the number of P. aeruginosa PA14 cells in tumors at lower concentrations. Hence, we present a versatile experimental system that is providing a platform to test approved and newly developed antibiofilm compounds.
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In Vivo Efficacy of Antimicrobials against Biofilm-Producing Pseudomonas aeruginosa. 2015, 59 (8):4974-81 Antimicrob. Agents Chemother.
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en
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1098-6596