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FIBER OPTIC BIOSENSING BASED ON RECEPTOR RECOGNITION

Wolfbeis, Otto S.
; 
He, Huarui
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Issue Date
1992
Submitted date
2024-05-08
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Abstract
Enzymes,ion carriers, and optically active receptors are shownto be useful for specific recognition and sensing of biologically important species including enzymesubstrates, metabolites, and drugs. In enzyme-based sensors, optical assays for substrates (such as glucose, lactate or cholesterol)have bee designed andare fairly established. The specificity of such sensorsis usually very high. However, quite a numberof assays cannot be performed with enzymes, and use has to be made ofion carriers and other recognition elements. A quite different situation is found in such cases. In contrast to enzymes which "digest" their substrates, receptors do not metabolize their substrates. They therefore offer an promising alternative for enzymatic recognition of substrates. However, most existing receptors lack the tremendousspecificity of enzymes. Neutral on carriers which may be considered as a kind of ion receptors, are a notable exception. If the receptoris enantio-selective(i.e. preferably binds one speciesoutofa pairof optical isomers)a fairly specific recognition of enantiomers of biogenic amines (such as some drugs and biogenic amines) becomes possible. In contrast to enzyme-based sensing where steady-state response is a result of kinetic equilibration, this type of substrate binding results in thermodynamicequilibration. Specific examples are given for new types sensors and sensors based on recognition by enzymes, neutralion carriers, charge-transfer interaction, and hydrogen bonding. Their respective merits and limitations are discussed.
Citation
Biosensors : fundamentals, technologies and applications, 425 - 434
Affiliation
Institute for Optical Sensors, Joanneum Research, Steyrer-Gasse 17, A-8010 Graz, Austria; Institute of Organic Chemistry, Karl-Franzens-University, Heinrichstr. 28, A-8010 Graz, Austria
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623829
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Type
Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International
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