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Single-nucleotide polymorphism-based genetic diversity analysis of clinical Pseudomonas aeruginosa isolates.
Muthukumarasamy, Uthayakumar Preusse, Matthias Kordes, Adrian Koska, Michal Schniederjans, Monika Khaledi, Ariane Häussler, Susanne
Muthukumarasamy, Uthayakumar
Preusse, Matthias
Kordes, Adrian
Koska, Michal
Schniederjans, Monika
Khaledi, Ariane
Häussler, Susanne
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2020-03-20
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Extensive use of next-generation sequencing has the potential to transform our knowledge on how genomic variation within bacterial species impacts phenotypic versatility. Since different environments have unique selection pressures, they drive divergent evolution. However, there is also parallel or convergent evolution of traits in independent bacterial isolates inhabiting similar environments. The application of tools to describe population-wide genomic diversity provides an opportunity to measure the predictability of genetic changes underlying adaptation. Here we describe patterns of sequence variations in the core genome among 99 individual Pseudomonas aeruginosa clinical isolates and identified single nucleotide polymorphisms (SNPs) that are the basis for branching of the phylogenetic tree. We also identified SNPs that were acquired independently, in separate lineages, and not through inheritance from a common ancestor. While our results demonstrate that the P. aeruginosa core genome is highly conserved and in general, not subject to adaptive evolution, instances of parallel evolution will provide an opportunity to uncover genetic changes that underlie phenotypic diversity.
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Genome Biol Evol. 2020 Mar 20. pii: 5810496. doi: 10.1093/gbe/evaa059.
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en
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1759-6653
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Attribution-NonCommercial-ShareAlike 4.0 International