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CONSTRUCTION OF NEW EXPRESSION VECTORS FOR MAMMALIAN CELLS USING THE IMMEDIATE EARLY ENHANCER OF THE HUMAN CYTOMEGALOVIRUS TO INCREASE EXPRESSION FROM HETEROLOGOUS ENHANCER/ PROMOTERS
Wirth, Manfred Schumacher, Lorin Hauser, Hansjörg
Wirth, Manfred
Schumacher, Lorin
Hauser, Hansjörg
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Issue Date
1991
Submitted date
2024-02-20
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Abstract
Mammalian expression vectors were optimized by the addition of a human immediate early (hCMVIE) enhancer DNA fragment in cis to heterologous enhancer/promoters. The hCMVIE enhancer fragment mediated a 3-5 fold increase in stable, but not in transient expression from SV40 expression plasmids ina position dependent, but orientation independent manner. Maximum expression in BHK cells was obtained region by separating the hCMVIE enhancer 2 kb from a target SV40 promoter/enhancer. The effect of the hCMVIE fragment is not restricted to the SV40 system and is also observed with expression plasmids harboring the MPSv retroviral LTR. An up to eightfold increase in luciferase expression is obtained after stable transfection of CMV/MPSV-expression constructs if compared to the respective SV40 expression plasmid.
Citation
Protein glycosylation, 49 - 52
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 15
ISSN
0930-4320
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ISBN
1560811846
3527283676
3527283676
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Attribution-NonCommercial-ShareAlike 4.0 International