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On Line Determination of Glucose Concentration in Animal Cell Cultures Based on Chemiluminescent Detection Coupled with Flow Injection Analysis
Huang, Y. L. Li, S.-Y. Dremel, Bernd A. A. Bilitewski, Ursula Schmid, Rolf D.
Huang, Y. L.
Li, S.-Y.
Dremel, Bernd A. A.
Bilitewski, Ursula
Schmid, Rolf D.
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Issue Date
1991
Submitted date
2024-01-31
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Abstract
A flow injection analysis (FIA) system for the on-line determination of glucose in animalcell cultures is described. The hydrogen peroxide generated in the enzyme reaction is determined via a highly sensitive chemiluminescent reaction with luminol. Based on the measurement of the maximum emitted light intensity, the system was able to analyse glucose in a linear range of 10° to 5x 102 M glucose concentration, with an r.s.d. of 3% at the 1 mM level (5 measurements). The enzyme reactor used was stable for more than 4 weeks in continous operation. The FIA developed wasable to analyse up to 20 samples per hour. The system has been sucessfully applied to on-line monitoring of glucose concentration during an animal cell culture, designed for the production of human antithrombin III factor. Results obtained with the FIA system were compared with off-line results, obtained with a Yellow Springs Instrument Company Model27 (YSI).
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 77 - 80
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
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ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International