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ENZYME-CATALYZED DETERMINATION OF 2-OXOISOCAPROATE BY FLOW-INJECTION ANALYSIS AND ITS APPLICATION TO THE MONITORING FOR MAPLE SYRUP URIN DISEASE
Tauschensky, S. Hummel, W. Schadewaldt, P. Wendel, U.
Tauschensky, S.
Hummel, W.
Schadewaldt, P.
Wendel, U.
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Issue Date
1991
Submitted date
2024-01-31
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Abstract
A simple and fast method for the determination of 2—oxoisocaproate in plasma samples by use of NAD*-dependent D-2-hydroxyisocaproate dehydrogenase (Hic-DH) from Lactobacillus casei ssp. is described. The enzyme is immobilized on aminopropyl-CPG and applicated in a flow-injection system with detection of NADH absorbance. Applicability is demonstrated by comparative measurement of 2-0xoisocaproate levels in plasma samples of patients with maple syrup urine disease by the photometric method and the flow-injection analysis.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 145 - 148
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
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ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International