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Purification and Kinetic Studies of Lipases Using Reversed Micellar Systems
Aires-Barros, M. R. Cabral, J. M. S.
Aires-Barros, M. R.
Cabral, J. M. S.
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Issue Date
1991
Submitted date
2024-03-27
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Abstract
Selective separation and purification of two lipases (A and B) from Chromobacterium viscosum were carried out by liquid-liquid extraction using a reversed micellar system (250 mM AOTin isooctane), with selective extraction of lipase B to the micellar phase and lipase A remaining in aqueous solution. The purified lipases were used to catalyze esterification reactions between carboxylic acids and alcohols in reversed micelles. The influence of various parameters in the synthesis of oleyl propionate by lipases A and B, was studied. The maximum activity was obtained at Wo([H20]/[AOT])=6.7.
Citation
Lipases : structure, mechanism and genetic engineering, 407 - 416
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International