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Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB.
Groenewold, Maike K Hebecker, Stefanie Fritz, Christiane Czolkoss, Simon Wiesselmann, Milan Heinz, Dirk W Jahn, Dieter Narberhaus, Franz Aktas, Meriyem Moser, Jürgen
Groenewold, Maike K
Hebecker, Stefanie
Fritz, Christiane
Czolkoss, Simon
Wiesselmann, Milan
Heinz, Dirk W
Jahn, Dieter
Narberhaus, Franz
Aktas, Meriyem
Moser, Jürgen
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2019-01-01
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Abstract
Agrobacterium tumefaciens transfers oncogenic T-DNA via the type IV secretion system (T4SS) into plants causing tumor formation. The acvB gene encodes a virulence factor of unknown function required for plant transformation. Here we specify AcvB as a periplasmic lysyl-phosphatidylglycerol (L-PG) hydrolase, which modulates L-PG homeostasis. Through functional characterization of recombinant AcvB variants, we showed that the C-terminal domain of AcvB (residues 232-456) is sufficient for full enzymatic activity and defined key residues for catalysis. Absence of the hydrolase resulted in ~10-fold increase in L-PG in Agrobacterium membranes and abolished T-DNA transfer and tumor formation. Overproduction of the L-PG synthase gene (lpiA) in wild-type A. tumefaciens resulted in a similar increase in the L-PG content (~7-fold) and a virulence defect even in the presence of intact AcvB. These results suggest that elevated L-PG amounts (either by overproduction of the synthase or absence of the hydrolase) are responsible for the virulence phenotype. Gradually increasing the L-PG content by complementation with different acvB variants revealed that cellular L-PG levels above 3% of total phospholipids interfere with T-DNA transfer. Cumulatively, this study identified AcvB as a novel virulence factor required for membrane lipid homeostasis and T-DNA transfer.
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Mol Microbiol. 2019 Jan;111(1):269-286. doi: 10.1111/mmi.14154. Epub 2018 Nov 14.
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en
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1365-2958
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Attribution-NonCommercial-ShareAlike 4.0 International