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DEVELOPMENT OF AN ENZYMATIC MULTICHANNEL FLOW INJECTION ANALYSIS SYSTEM FOR MONITORING MAMMALIAN CELL FERMENTATIONS
Van der Pol, J. J. Joksch, B. Eberhardt, R. Biselli, M. Wandrey, Ch. Tramper, J.
Van der Pol, J. J.
Joksch, B.
Eberhardt, R.
Biselli, M.
Wandrey, Ch.
Tramper, J.
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Issue Date
1992
Submitted date
2024-05-08
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Abstract
A multichannel flow injection analysis system described earlier /1/, is adapted to mammalian cell fermentations by increasing the sensitivity of the system. The analysis is based on the conversion of the compounds by immobilized dehydrogenases, resulting in the built up or consumption of NADH which is measured with a fluorescence- detector. The method presented here has a linear glucose determination range of 0.05 g/1 to 3.5 g/1 and a non-linear range for lactate of 0.05 g/1 to 2.7 g/l.
Citation
Biosensors : fundamentals, technologies and applications, 349 - 352
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International