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Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.
Mathieu, Eve-Lyne Finkernagel, Florian Murawska, Magdalena Scharfe, Maren Jarek, Michael Brehm, Alexander
Mathieu, Eve-Lyne
Finkernagel, Florian
Murawska, Magdalena
Scharfe, Maren
Jarek, Michael
Brehm, Alexander
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2012-06
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Abstract
The ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.
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Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. 2012, 40 (11):4879-91 Nucleic Acids Res.
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en
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1362-4962
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