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ELIMINATION OF INTERFERENCES IN GLUCOSE DETERMINATION IN BLOOD SERUM USING FLOW-INJECTION AMPEROMETRY

Trojanowicz, Marek
; 
Matuszewski, Wojciech
; 
Lewenstam, Andrzej
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Issue Date
1991
Submitted date
2024-01-31
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Abstract
A flow-injection system with anodic amperometric detection for glucose determination in human blood serum samples was optimized by a comparison of various arrangements of enzyme immobilization and elimination of interferences such as ascorbic acid, urea and uric acid. The best results were obtained using a compact membrane biosensor in wall-jet flow-through detector, where Pt disc electrode was covered with evaporated Nafion layer, then a polyester membrane with immobilized glucose oxidase and another protective polyester membrane. In the optimized conditions a linear response up to 25mM glucose was observed with detection limit 200uM glucose and sampling rate 120 hr-1 for 20p1 sample volume. Results of flow injection glucose determination in human serum samples were compared with Beckman Glucose Analyser 2 and Kone Dynamic analyser.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 159 - 163
Affiliation
Department of Chemistry, University of Warsaw, Pasteura l, 02-093 Warsaw, Poland; Department of Analytical Chemistry, Abo Akademi University, SF-20500 Turku, Finland
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991
URI
http://hdl.handle.net/10033/623626
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Type
Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
1560811218
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International
cbn
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