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Haprolid Inhibits Tumor Growth of Hepatocellular Carcinoma through Rb/E2F and Akt/mTOR Inhibition.
Xing, Jun Bhuria, Vikas Bui, Khac Cuong Nguyen, Mai Ly Thi Hu, Zexi Hsieh, Chih-Jen Wittstein, Kathrin Wilkens, Ludwig Li, Jun
Xing, Jun
Bhuria, Vikas
Bui, Khac Cuong
Nguyen, Mai Ly Thi
Hu, Zexi
Hsieh, Chih-Jen
Wittstein, Kathrin
Wilkens, Ludwig
Li, Jun
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2020-03-06
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Abstract
The efficacy of haprolid was evaluated in human HCC cell lines (Huh-7, Hep3B and HepG2) and xenograft tumors (NMRI-Foxn1nu mice with injection of Hep3B cells). Cytotoxic activity of haprolid was determined by the WST-1 and crystal violet assay. Wound healing, transwell and tumorsphere assays were performed to investigate migration and invasion of HCC cells. Apoptosis and cell-cycle distribution were measured by flow cytometry. The effects of haprolid on the Rb/E2F and Akt/mTOR pathway were examined by immunoblotting and immunohistochemistry.
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Cancers (Basel). 2020 Mar 6;12(3). pii: cancers12030615. doi: 10.3390/cancers12030615.
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Article
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en
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2072-6694
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Attribution-NonCommercial-ShareAlike 4.0 International