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EXTRINSIC FIBER OPTIC BIOSENSORS WITH LIGHT EMITTING ENZYME SYSTEMS
Coulet, P. R. Blum, L. J. Gautier, S. M.
Coulet, P. R.
Blum, L. J.
Gautier, S. M.
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Issue Date
1992
Submitted date
2024-05-08
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Adobe PDF, 2.92 MB
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Abstract
A multipurpose fiber-optic sensor was designed in our group, with chemi- or bioluminescent enzyme systems boundonto a preactivated nylon membraneattached to the end of 8-mm diameter glass fiber bundle. This waveguide was connected to the PMTof a luminometer enabling remote detection ofspecific target analytes. The fiber-optic sensor could be used for H7O7 measurements with immobilized peroxidase. The maximum lightintensity was obtained within 1 min and measurements could be performed in the range 2 x10°8 M - 2 x10-5 M. With immobilized firefly luciferase, ATP measurements could be performed over a wide linear range from 2 x 10-11 Mto 1 x 10-6 M.With the bacterial oxidoreductase-luciferase system, the linear dynamic range was comprised between 1 x 10-9 M and 3 x 10-6 M. Theuseofthe biosensor has been extended to other analyteslike sorbitol, ethanol and oxaloacetate using specific dehydrogenases co-immobilized with the bacterial enzyme system. The alternate determination of ATP and NADH with the samesensor using the two co-immobilised bioluminescent systems on the same membrane was also developed using reaction mediums compatible with both the activity and stability of each enzyme. The combination of FIA with these biosensors has also been explored and a reagentless biosensor with co-reactants embedded andreleased in the immediate vicinity of the sensing tip is now under development.
Citation
Biosensors : fundamentals, technologies and applications, 419 - 424
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International