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ASCORBIC ACID DETERMINATION IN FRUIT JUICES BASED ON A FIBRE OPTIC ASCORBIC ACID BIOSENSOR AND FLOW INJECTION ANALYSIS
Schaffar, Bernhard P. H. Dremel, Bernd A. A. Schmid, Rolf D.
Schaffar, Bernhard P. H.
Dremel, Bernd A. A.
Schmid, Rolf D.
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
A new method using a fibre optic ascorbic acid biosensor as the detection unit in a flow injection analysis (FIA) system is presented. The biosensor is based on a fibre optic oxygen optrode which measures the oxygen consumption via dynamic quenching of the fluorescence of a dye by molecular oxygen. Ascorbic acid oxidase is immobilised onto the surface of the oxygen optrode as biological component. To detect ascorbic acid in fruit juices it is necessary to protect the biosensor from low pH, such as is present in fruit juices (e.g. lemon juice pH 1.5), using a FIA-system which automatically buffers and dilutes the juice samples.
Citation
Biosensors : applications in medicine, environmental protection and process control, 229 - 232
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
0895739550
3527280324
3527280324
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Attribution-NonCommercial-ShareAlike 4.0 International