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Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases.
Gemperlein, Katja Dietrich, Demian Kohlstedt, Michael Zipf, Gregor Bernauer, Hubert S Wittmann, Christoph Wenzel, Silke C Müller, Rolf
Gemperlein, Katja
Dietrich, Demian
Kohlstedt, Michael
Zipf, Gregor
Bernauer, Hubert S
Wittmann, Christoph
Wenzel, Silke C
Müller, Rolf
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2019-09-06
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Long-chain polyunsaturated fatty acids (LC-PUFAs), particularly the omega-3 LC-PUFAs eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), have been associated with beneficial health effects. Consequently, sustainable sources have to be developed to meet the increasing demand for these PUFAs. Here, we demonstrate the design and construction of artificial PUFA biosynthetic gene clusters (BGCs) encoding polyketide synthase-like PUFA synthases from myxobacteria adapted for the oleaginous yeast Yarrowia lipolytica. Genomic integration and heterologous expression of unmodified or hybrid PUFA BGCs yielded different yeast strains with specific LC-PUFA production profiles at promising yield and thus valuable for the biotechnological production of distinct PUFAs. Nutrient screening revealed a strong enhancement of PUFA production, when cells were phosphate limited. This represents, to the best of our knowledge, highest concentration of DHA (16.8 %) in total fatty acids among all published PUFA-producing Y. lipolytica strains.
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Nat Commun. 2019 Sep 6;10(1):4055. doi: 10.1038/s41467-019-12025-8.
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2041-1723
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Attribution-NonCommercial-ShareAlike 4.0 International