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ENGINEERING PROTEINS FOR BIOSENSOR APPLICATIONS
Ulmer, Kevin M.
Ulmer, Kevin M.
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Issue Date
1987
Submitted date
2023-08-30
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Abstract
The rapidly developing field of protein engineering will soon make it possible to tailor the properties of enzymes, antibodies and other proteins for the fabrication of a new generation of biosensors. Problems which have previously limited the broader commercial application of biosensors, such as stability and immobilization efficiency, are likely to be overcome by the successful application of this new technology. Molecular biologists are now able to rapidly and efficiently alter the amino acid sequence of any protein for which the gene has been previously cloned or synthesized. When such changes are based upon detailed knowledge of the threedimensional atomic structure of the protein and of its biochemical properties, it is possible to alter both the structure and function of the protein in a rational, engineering fashion. Early success has already been reported in altering stability, pH profile, specificity and kinetic parameters. Protein engineering is now also being used to address the central problem of attaching proteins to the inorganic components of biosensors in order to enhance our control over the orientation, arrangement and molecular details of immobilization. When such an approach is combined with surface chemical modification and monolayer techniques, it should be possible to achieve far greater control over the critical properties of the interface between the active protein and the signal transducing portion of the sensor.
Citation
Biosensors International Workshop 1987, 245 - 250
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
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ISBN
0-89573-683-7
3-527-26801-4
3-527-26801-4
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Attribution-NonCommercial-ShareAlike 4.0 International