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CLONING AND EXPRESSION OF PROTEINS FROM THERMUS THERMOPHILUS IN ESCHERICHIA COLI
Weisshaar, Maria-Paz Sprinzl, Mathias
Weisshaar, Maria-Paz
Sprinzl, Mathias
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
Cloning and expression of genesfrom the extreme thermophilic bacterium Thermus thermophilus in the mesophilic Escherichia coli was investigated. Weidentified the structural genes (tuf1, tuf2) of the protein elongation factor Tu from Thermus thermophilus and determined the nucleotide sequenceof tuf1. The expression of tuf1 occurs in E. coli from its T. thermophilus own promoter. The organization of the T. thermophilus operon which contains tuf1 is similar to the homologous operonin E. coli. The codon usage-of the T. thermophilus genesis different from that in E. coli. Codons with a guanine in the third position are preferentially usedin this thermophilic organism. The codon usage is mostly determined by the necessity to stabilize the codon-anticodoninteraction at high temperature.
Citation
Biosensors : applications in medicine, environmental protection and process control, 417 - 419
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International