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AUTOMATED LIPOSOME-BASED FLOW INJECTION IMMUNOASSAY SYSTEM
Durst, Richard E. Locascio-Brown, Laurie Plant, Anne L.
Durst, Richard E.
Locascio-Brown, Laurie
Plant, Anne L.
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
A liposome-based flow injection immunoassay (FIIA) system for quantitation ofa clinical analyte, theophylline, and also, with very minor changes in the assay format, of an antibody to theophylline, has been developed. Automated sequential analyses were performed at room temperaturewith picomolesensitivity and a day-to-day coefficient of variation of less than 5 %. The system components include liposomesthat contain fluorophoresin their aqueouscentral cavities and an immobilized-antibody reactor column. The immunoreactor was regenerated hundreds of times over three monthsof continuoususe with no measurable loss of antibody activity. The special advantages ofusing flow injection analysis for immunoassays and ofusing liposomesin FIIA, compared to most current immunoassay techniques,are described.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 181 - 190
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International