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APPLICATIONS AND KINETICS OF IMMOBILIZED ENZYMES AND COUPLED ENZYME REACTIONS
Dominguez, Elena Marko-Varga, György Hahn-Hägerdal, Bärbel Gorton, Lo
Dominguez, Elena
Marko-Varga, György
Hahn-Hägerdal, Bärbel
Gorton, Lo
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
Immobilization of enzymesresults in more adequate reagentsto be used analytically. After immobilization, the kinetic parameters of the enzymes are modified and nothing can be predicted abouttheactivity of the heterogeneous system. In coupled enzymereactions with co-immobilized enzymes, this is even more important owingto the kinetic dependence on each consecutive reaction. The determination of ethanol and acetaldehyde is considered in this paper, using two different coupled enzyme systems. Some important parameters, as the enzyme charged,the ratio of each enzymein the sequence and the immobilization yield, are considered in terms of conversion efficiency finally determiningthesensitivity of the analysis.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 225 - 228
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
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ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International