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OCTN2-mediated acetyl-l-carnitine transport in human pulmonary epithelial cells in vitro
Salomon, Johanna J. Salomon, J. Gausterer, Julia C. Selo, Mohammed Ali Hosoya, Ken Ichi Huwer, Hanno Schneider-Daum, Nicole Lehr, Claus Michael Ehrhardt, Carsten
Salomon, Johanna J.
Salomon, J.
Gausterer, Julia C.
Selo, Mohammed Ali
Hosoya, Ken Ichi
Huwer, Hanno
Schneider-Daum, Nicole
Lehr, Claus Michael
Ehrhardt, Carsten
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2019-08-01
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Abstract
The carnitine transporter OCTN2 is associated with asthma and other inflammatory diseases. The aims of this work were (i) to determine carnitine uptake into freshly isolated human alveolar type I (ATI)-like epithelial cells in primary culture, (ii) to compare the kinetics of carnitine uptake between respiratory epithelial in vitro cell models, and (iii) to establish whether any cell line was a suitable model for studies of carnitine transport at the air-blood barrier. Levels of time-dependent [3H]-acetyl-l-carnitine uptake were similar in ATI-like, NCl-H441, and Calu-3 epithelial cells, whereas uptake into A549 cells was ~5 times higher. Uptake inhibition was more pronounced by OCTN2 modulators, such as l-Carnitine and verapamil, in ATI-like primary epithelial cells compared to NCl-H441 and Calu-3 epithelial cells. Our findings suggest that OCTN2 is involved in the cellular uptake of acetyl-l-carnitine at the alveolar epithelium and that none of the tested cell lines are optimal surrogates for primary cells.
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Pharmaceutics. 2019 Aug 7;11(8). pii: pharmaceutics11080396. doi: 10.3390/pharmaceutics11080396.
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en
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8
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Attribution-NonCommercial-ShareAlike 4.0 International