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FIA based on Enzymes and Antibodies - developments at the GBF
Schmid, Rolf D.
Schmid, Rolf D.
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
A major feature of the GBFis its capacity to carry out bioprocess development based on microorganisms, recombinant microorganisms and animalcells in the 300 | to 5000 | scale. As a result, the GBF proved a fertile testing ground for the development of bioprocess analysis. The first attempt to employ FIA methods in this context was reported by GBFresearchers in 1985 (1-3). Two years later, a major program in biosensor development was launched which now not only comprised bioprocess control but also analytical procedures for food and environmental samples. Though concepts for disposable sensors were an integral part of this program from thestart, flow injectionbased procedures received particular attention, since FIA based on enzymes and antibodies proved a powerful tool for the development of those analytical protocols designed for semi-continuous operation and/or the analysis of large numbers of samples. In addition, FIA offers several advantages compared with other biosensor concepts; thus, the linear detection range of biosensors, which is often quite limited, can be largely extended in FIA, through the method of "zone sampling", and if two analytical reaction sequences of largely different pH optima have to be combined, FIA mightoften beleft as the only possibility. In the following, FIA-related work at the GBF over the past 6 years in the areas of bioprocess control, downstream processing, food and pesticide analysis is summarized.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 297 - 303
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
1560811218
3527282491
3527282491
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Gov't Doc #
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Attribution-NonCommercial-ShareAlike 4.0 International