Loading...
Establishment of a highly sensitive assay for detection of Hepatitis E virus-specific immunoglobulins.
Bohm, Katrin Strömpl, Julia Krumbholz, Andi Zell, Roland Krause, Gérard Sievers, Claudia
Bohm, Katrin
Strömpl, Julia
Krumbholz, Andi
Zell, Roland
Krause, Gérard
Sievers, Claudia
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2019-11-06
Submitted date
Files
Loading...
accepted manuscript
Adobe PDF, 1.35 MB
Other Titles
Abstract
Hepatitis E, a liver disease caused by infection with the hepatitis E virus (HEV) is a worldwide emerging disease. The diagnosis is based on the detection of viral RNA and of HEV-specific immunoglobulins (Ig). For the latter, various assays are commercially available but still lack harmonization.In this study, a Luminex®-based multiplex serological assay was established, that measures the presence of total IgG, IgA and IgM antibodies, targeting a short peptide derived from the viral E2 protein. For the validation, 160 serum samples with a known HEV serostatus were used to determine the assay cut-off and accuracy. Thereby, HEV-IgG and -RNA positive sera were identified with a sensitivity of 100% and a specificity of 98% [CI95% 94-100%].Application of the assay by re-testing 514 sera previously characterised with different HEV-IgG or total antibody tests, revealed a high level of agreement between the assays (Cohens Kappa 0.58-0.99).The established method is highly sensitive, specific and can be easily implemented in a multiplex format to facilitate rapid differential diagnostics with a few microliters of sample input.
Citation
J Clin Microbiol. 2019 Nov 6. pii: JCM.01029-19. doi: 10.1128/JCM.01029-19
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1098-660X
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International